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Última A protein with amino acid sequence homology to bovine insulin is present in the legume Vigna unguiculata (cowpea) BJMBR Venâncio,T.M.; Oliveira,A.E.A.; Silva,L.B.; Machado,O.L.T.; Fernandes,K.V.S.; Xavier-Filho,J.. Since the discovery of bovine insulin in plants, much effort has been devoted to the characterization of these proteins and elucidation of their functions. We report here the isolation of a protein with similar molecular mass and same amino acid sequence to bovine insulin from developing fruits of cowpea (Vigna unguiculata) genotype Epace 10. Insulin was measured by ELISA using an anti-human insulin antibody and was detected both in empty pods and seed coats but not in the embryo. The highest concentrations (about 0.5 ng/µg of protein) of the protein were detected in seed coats at 16 and 18 days after pollination, and the values were 1.6 to 4.0 times higher than those found for isolated pods tested on any day. N-terminal amino acid sequencing of insulin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Insulin; Insulin-like; Cowpea; Vigna unguiculata; Developing fruits; Sequence homology; Evolution. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900004 Apparent cross-talk of two signaling pathways that regulate Zea mays coleoptile growth Phyton Buentello Volante,B; Díaz de León-Sánchez,F; Rivera-Cabrera,F; Aguilar Caballero,R; Ponce-Valadez,M; Sánchez de Jiménez,E; Pérez-Flores,LJ. Auxin and insulin promote Zea mays embryo growth, induce S6 ribosomal protein (S6rp) phosphorylation, and promote specific protein synthesis. The objective of this research was to test a possible cross-talk between insulin and auxin transduction pathways in Z. mays coleoptiles, typical auxin target tissues. Auxin and insulin produced differential quantitative and qualitative stimulation of cytoplasmic and ribosomal protein phosphorylation, and specific patterns of de novo synthesized cytoplasmic proteins. In addition, insulin induced S6rp phosphorylation was strongly inhibited by rapamycin, indicating target of rapamycin (TOR) kinase participation; auxin-induced S6rp phosphorylation was insensitive to this inhibitor. Phosphatidic acid (PA), a second... Tipo: Info:eu-repo/semantics/article Palavras-chave: Auxins; Insulin; Phosphatidic acid; ZmS6K; TOR; Plant growth. Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000100015 Association of postalimentary lipemia with atherosclerotic manifestations BJMBR Tentor,J.; Nakamura,R.T.; Gidlund,M.; Barros-Mazon,S.; Harada,L.M.; Zago,V.S.; Oba,J.F.; Faria,E.C. de. We identified different lipemic and metabolic responses after the ingestion of a standardized meal by healthy adults and related them to atherosclerotic markers. Samples from 60 normolipidemic adults were collected before and after a liquid meal (40 g fat/m² body surface) at 0, 2, 4, 6, and 8 h for measurements of lipids, free fatty acids (FFA), insulin, cholesteryl ester transfer protein (CETP), autoantibodies to epitopes of oxidized LDL (oxLDL Ab), lipolytic activities, and apolipoprotein E polymorphism. Mean carotid intima-media thickness (cIMT) was determined by Doppler ultrasound. The volunteers were classified into early (N = 39) and late (N = 31) triacylglycerol (TAG) responders to the test meal. Late responders showed lower HDL cholesterol... Tipo: Info:eu-repo/semantics/article Palavras-chave: Postalimentary lipemia; Autoantibodies to epitopes of oxidized LDL; Carotid intima-media thickness; Insulin; Free fatty acids. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100014 Biological response of spontaneously hypertensive rats to the streptozotocin administration BABT Costa,Virginia Alice Vieira da; Vianna,Lucia Marques. The sensitivity of adult spontaneously hypertensive rats (SHR) to the diabetogenic effect of streptozotocin (STZ) was studied. The animals were subdivided into three groups: control (citrate buffer), streptozotocin 40 mg/kg or 50 mg/kg, and general biologic parameters were analyzed, in addition to systolic blood pressure, blood glucose and insulin levels determinations. Both doses were able to induce hyperglycemia above 300 mg/dl; however, 50 mg/kg provoked a more pronounced physiological alterations in body weight, diuresis, water and food intake. There was no change on systolic blood pressure with either dose. Results suggested that SHRs did not need doses of streptozotocin above 40mg/kg in order to produce diabetes probably because this strain was much... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptozotocin; SHR; Diabetes; Blood pressure; Insulin. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100006 Biphasic modulation of insulin receptor substrate-1 during goitrogenesis BJMBR Grozovsky,R.; Morales,M.M.; Carvalho,D.P.. Insulin receptor substrate-1 (IRS-1) is the main intracellular substrate for both insulin and insulin-like growth factor I (IGF-I) receptors and is critical for cell mitogenesis. Thyrotropin is able to induce thyroid cell proliferation through the cyclic AMP intracellular cascade; however, the presence of either insulin or IGF-I is required for the mitogenic effect of thyroid-stimulating hormone (TSH) to occur. The aim of the present study was to determine whether thyroid IRS-1 content is modulated by TSH in vivo. Strikingly, hypothyroid goitrous rats, which have chronically high serum TSH levels (control, C = 2.31 ± 0.28; methimazole (MMI) 21d = 51.02 ± 6.02 ng/mL, N = 12 rats), when treated with 0.03% MMI in drinking water for 21 days, showed... Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid; Goiter; Insulin receptor substrate; Insulin; Thyrotropin. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500011 Consequences of cerebroventricular insulin injection on renal sodium handling in rats: effect of inhibition of central nitric oxide synthase BJMBR Oliveira,P.C.; Michelotto,J.B.; Zapparoli,A.; Boer,P.A.; Gontijo,J.A.R.. In the present study, we investigated the effects of acute intracerebroventricular (icv) insulin administration on central mechanisms regulating urinary sodium excretion in simultaneously centrally NG-nitro-L-arginine methylester (L-NAME)-injected unanesthetized rats. Male Wistar-Hannover rats were randomly assigned to one of five groups: a) icv 0.15 M NaCl-injected rats (control, N = 10), b) icv dose-response (1.26, 12.6 and 126 ng/3 µL) insulin-injected rats (N = 10), c) rats icv injected with 60 µg L-NAME in combination with NaCl (N = 10) or d) with insulin (N = 10), and e) subcutaneously insulin-injected rats (N = 5). Centrally administered insulin produced an increase in urinary output of sodium (NaCl: 855.6 ± 85.1 Δ%/min; 126 ng insulin: 2055 ± 310.6... Tipo: Info:eu-repo/semantics/article Palavras-chave: Central nervous system; Intracerebroventricular; Nitric oxide inhibition; Insulin; Natriuresis; Lithium clearance. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200013 Diet plus insulin compared to diet alone in the treatment of gestational diabetes mellitus: a systematic review BJMBR Giuffrida,F.M.A.; Castro,A.A.; Atallah,A.N.; Dib,S.A.. Fetuses of mothers with gestational diabetes mellitus are at increased risk to develop perinatal complications mainly due to macrosomia. However, in view of the marked heterogeneity of this disease, it seems difficult to set guidelines for diagnosis and treatment. This complicates the choice of assigning patients either to diet or to insulin therapy. Also of concern is how much benefit could be expected from insulin therapy in preventing fetal complications in these patients. In a systematic review of the literature assessing the efficacy of insulin in preventing macrosomia in fetuses of mothers with gestational diabetes, we found six randomized controlled trials comparing diet alone to diet plus insulin. The studies included a total of 1281 patients (644... Tipo: Info:eu-repo/semantics/other Palavras-chave: Diabetes; Gestational; Diet therapy; Fetal macrosomia; Insulin; Meta-analysis. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000004 Dietary vegetable oils inclusion on the performance, hormonal levels and hsp 70 gene expression in broilers under heat stress Animal Sciences Tari, Ali Rafiei; Sadeghi, Ali Asghar; Mousavi, Seyed Naser. The aim of the present study was to compare the effects of unsaturated and saturated oils on the performance, hormonal levels and hsp gene expression in broiler chickens exposed to heat stress. 300 one-day male broiler chicks were assigned to 4 treatments (Diets containing palm, corn, linseed or olive oils) with 5 replicates. At day 28 of age, 2 chickens were removed from each replicate, then blood samples and liver tissue samples were collected for analyses. Feeding linseed and olive oil reduced feed conversion ratio compared to corn and palm oils. The lowest level of insulin was for chickens fed linseed oil and corn oil. The highest level of corticosterone was found in chickens fed palm oil and the lowest level was for those received linseed oil.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Corticosterone; Insulin; Heat shock protein; Heat tolerance. Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/45517 Efecto antidiabético de los polisacáridos del fruto de xoconostle (Opuntia matudae) en ratas. Colegio de Postgraduados Calvo Arriaga, Ayne Oliva. La diabetes mellitus afecta a más de 220 millones de personas en el mundo y es una de las causas principales de muerte. Existe evidencia de que los frutos ácidos de Opuntia joconostle y O. matudae, denominados xoconostle, disminuyen la glucemia e incrementan la concentración sérica de insulina en individuos diabéticos. El presente estudio fue propuesto para dilucidar si esos efectos y otros relacionados con el metabolismo energético, como la disminución de colesterol y triglicéridos en la sangre, se deben a los mucílagos, pectinas o hemicelulosas de xoconostle, a todos ellos o a ninguno de ellos. La hipótesis es que estos polisacáridos ocasionan disminución de las concentraciones séricas de glucosa, colesterol y triglicéridos, e incremento de la secreción... Palavras-chave: Dislipidemia; Fibra alimentaria; Glucemia; Insulina; Dietary fiber; Dyslipidemia; Glycemia; Insulin; Maestría; Botánica. Ano: 2011 URL: http://hdl.handle.net/10521/526 Efecto antidiabético de los polisacáridos del fruto de xoconostle (Opuntia matudae) en ratas. Colegio de Postgraduados Calvo Arriaga, Ayne Oliva. La diabetes mellitus afecta a más de 220 millones de personas en el mundo y es una de las causas principales de muerte. Existe evidencia de que los frutos ácidos de Opuntia joconostle y O. matudae, denominados xoconostle, disminuyen la glucemia e incrementan la concentración sérica de insulina en individuos diabéticos. El presente estudio fue propuesto para dilucidar si esos efectos y otros relacionados con el metabolismo energético, como la disminución de colesterol y triglicéridos en la sangre, se deben a los mucílagos, pectinas o hemicelulosas de xoconostle, a todos ellos o a ninguno de ellos. La hipótesis es que estos polisacáridos ocasionan disminución de las concentraciones séricas de glucosa, colesterol y triglicéridos, e incremento de la secreción... Palavras-chave: Dislipidemia; Fibra alimentaria; Glucemia; Insulina; Dietary fiber; Dyslipidemia; Glycemia; Insulin; Maestría; Botánica. Ano: 2011 URL: http://hdl.handle.net/10521/526 Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes BJMBR Latha,M.; Pari,L.. The effects of an aqueous extract of the plant Scoparia dulcis (200 mg/kg) on the polyol pathway and lipid peroxidation were examined in the liver of streptozotocin adult diabetic male albino Wistar rats. The diabetic control rats (N = 6) presented a significant increase in blood glucose, sorbitol dehydrogenase, glycosylated hemoglobin and lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides, and a significant decrease in plasma insulin and antioxidant enzymes such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) compared to normal rats (N = 6). Scoparia dulcis plant extract (SPEt, 200 mg kg-1 day-1) and glibenclamide (600 µg kg-1 day-1), a reference drug, were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione peroxidase; Glutathione-S-transferase; Insulin; Lipid peroxidation; Scoparia dulcis; Sorbitol dehydrogenase; Diabetes. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400015 Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice BJMBR Ferreira,M.C.L.; Lima,L.N.; Cota,L.H.T.; Costa,M.B.; Orsi,P.M.E.; Espíndola,R.P.; Albanez,A.V.; Rosa,B.B.; Carvalho,M.G.S.; Garcia,J.A.D.. The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. The LDLr-/- mice were divided into four experimental groups: Group C: standard feed; Group CT: standard feed and three teas, Group HL: high-fat feed; HLT Group: high-fat feed and three teas. The three types of tea (green, red, and white) originated from different processing of the Camellia sinensis L. plant, and were administered associated once a day at a dose of 25 mg/kg by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipids; Cardiovascular system; Tea; Glucose; Insulin. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500607 Effect of different levels of total dietary fiber on the performance of sows in gestation and lactation R. Bras. Zootec. Oelke,Carlos Alexandre; Ribeiro,Andréa Machado Leal; Noro,Mirela; Bernardi,Mari Lourdes; Denardin,Cristiane Casagrande; Nunes,Paula Raymundo; Veit,Fernando Cézar; Winckler,João Carlos. ABSTRACT The present study evaluated the effect of variation in the amount of total dietary fiber (TDF) in sow diets, provided from 74 to 114 days of gestation, on the digestibility of nutrients and energy during gestation, as well as productivity and metabolism during gestation and lactation. Thirty-three sows were used in a randomized experimental design. The amounts of TDF in three treatment diets were 156, 223, and 282 g kg−1. The daily intake of nutrients and energy was similar for all diets the entire period of gestation, differing only in the volume of ration consumed, which was 2.1, 2.2, and 2.4 kg day−1 (days 74 to 90) and 2.5, 2.6, and 2.8 kg day−1 (days 91 to 111) for the 156, 223, and 282 g kg−1 TDF diets, respectively. During gestation, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestibility; Insulin; Piglets. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100525 Effect of FSH and insulin on lipogenesis in cultures of Sertoli cells from immature rats BJMBR Follicle-stimulating hormone (FSH) and insulin regulate glycide metabolism in Sertoli cells, thus stimulating lactate production. These stimulatory effects of FSH and insulin do not require protein synthesis, suggesting a modulation of enzyme activity and/or regulation of glucose transport. The present investigation was performed to characterize the hormonal control of lipid metabolism in Sertoli cells. The data indicate that FSH and insulin have a regulatory effect on lipid metabolism in Sertoli cells. After 8 h of preincubation with insulin (5 µg/ml), the activity of the enzyme ATP-citrate lyase in cultured Sertoli cells was increased from 0.19 to 0.34 nmol NAD+ formed µg protein-1 min-1. FSH (100 ng/ml) had no effect on this enzyme. Glycerol phosphate... Tipo: Info:eu-repo/semantics/other Palavras-chave: Sertoli cells; Lipids; FSH; Insulin. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500004 Effect of high-fat diets on body composition, lipid metabolism and insulin sensitivity, and the role of exercise on these parameters BJMBR Coelho,D.F.; Pereira-Lancha,L.O.; Chaves,D.S.; Diwan,D.; Ferraz,R.; Campos-Ferraz,P.L.; Poortmans,J.R.; Lancha Junior,A.H.. Dietary fat composition can interfere in the development of obesity due to the specific roles of some fatty acids that have different metabolic activities, which can alter both fat oxidation and deposition rates, resulting in changes in body weight and/or composition. High-fat diets in general are associated with hyperphagia, but the type of dietary fat seems to be more important since saturated fats are linked to a positive fat balance and omental adipose tissue accumulation when compared to other types of fat, while polyunsaturated fats, omega-3 and omega-6, seem to increase energy expenditure and decrease energy intake by specific mechanisms involving hormone-sensitive lipase, activation of peroxisome proliferator-activated receptor α (PPARα) and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fat; Obesity; Insulin; PUFA; Diet; Exercise. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001000001 Effect of intracerebroventricularly injected insulin on urinary sodium excretion by cerebroventricular streptozotocin-treated rats BJMBR Macedo,R.F.; Furlan,F.C.; Marshall,P.S.; Michelotto,J.B.; Gontijo,J.A.R.. Recent evidence suggests that insulin may influence many brain functions. It is known that intracerebroventricular (icv) injection of nondiabetogenic doses of streptozotocin (STZ) can damage insulin receptor signal transduction. In the present study, we examined the functional damage to the brain insulin receptors on central mechanisms regulating glomerular filtration rate and urinary sodium excretion, over four periods of 30 min, in response to 3 µl insulin or 0.15 NaCl (vehicle) injected icv in STZ-treated freely moving Wistar-Hannover rats (250-300 g). The icv cannula site was visually confirmed by 2% Evans blue infusion. Centrally administered insulin (42.0 ng/µl) increased the urinary output of sodium (from 855.6 ± 85.1 to 2055 ± 310.6 delta%/min; N =... Tipo: Info:eu-repo/semantics/other Palavras-chave: Central nervous system; Insulin; Natriuresis; Streptozotocin; Lithium clearance. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900008 Endocrine cells and nerve ganglia of the small intestine of the Opossum Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae) Anais da ABC (AABC) Freitas-Ribeiro,Gláucia M.; Fonseca,Cláudio C.; Sartori,Sirlene S.R.; Loures-Ribeiro,Alan; Neves,Clóvis A.. The nervous and endocrine systems jointly control intestinal movements, secretions of their glands and also participate of the processes of nutrient digestion and absorption. Therefore, the central objective of this study was to verify the existence of a possible relationship between the number of nervous cells and ganglia of the submucosal and myenteric plexuses and the number of endocrine cells in the small intestine of adult D. aurita. The utilized staining techniques were Grimelius, modified Masson-Fontana, direct immunoperoxidase and H-E. Argyrophillic, argentaffin and insulin immunoreactive endocrine cells do not numerically vary between the initial, mid and final regions of the duodenum, jejunum and ileum (P>0.05), except for argyrophillic cells... Tipo: Info:eu-repo/semantics/article Palavras-chave: Argyrophillic cells; Argentaffin cells; Endocrine cells; Ganglia; Insulin. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300017 Ethnopharmacological activity of Hedera nepalensis K. Koch extracts and lupeol against alloxan-induced type I diabetes BJPS Hashmi,Waleed Javed; Ismail,Hammad; Jafri,Laila; Mirza,Bushra. In this study, we investigated the protective effects of Hedera nepalensis crude extract, its fractions and lupeol in alloxan-induced diabetic rats. Lupeol and n-hexane (HNN) fraction significantly reduced the blood glucose level by increasing insulin level in time dependent manner, and also significantly increased amylase and lipase activity in diabetic rats. Elevated levels of alanine transaminases (ALT), aspartate transaminases (AST), thiobarbituric acid reactive substances (TBARS), nitrite, hydrogen peroxide (H2O2), total bilirubin and total protein in blood serum were efficiently restored to normal levels. Suppressed enzymatic activity of catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and peroxidase (POD) were also restored to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidant; Amylase; Diabetes mellitus; Insulin; Hedera nepalensis; Lupeol. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100597 Grasa de sobrepaso en ovejas con diferente espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad. Colegio de Postgraduados Nieto Aquino, Rafael. El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg, n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se aplicaron 15 mg de PGF2α. No se encontraron diferencias (P > 0.05) para las... Palavras-chave: Ultrasonografía; Insulina; Progesterona; Estradiol; Hormona luteinizante. Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone.. Ano: 2012 URL: http://hdl.handle.net/10521/882 Grasa de sobrepaso en ovejas con diferente espesor de grasa dorsal y su respuesta en el estro sincronizado, perfil endocrino, porcentaje de gestación y prolificidad. Colegio de Postgraduados Nieto Aquino, Rafael. El objetivo de este estudio fue evaluar el efecto de la grasa de sobrepaso en el estro sincronizado, porcentaje de gestación y prolificidad en ovejas con baja y alta grasa dorsal, y su respuesta en hormona luteinizante (LH), estradiol (E2), progesterona (P4) e insulina (INS). Se utilizaron 59 ovejas, con diferente espesor de grasa dorsal, bajo (EGb) y alto (EGa), que se subdividieron en grupos: sin (EGbSg, n = 16) y con (EGbCg, n = 14) la adición de 150 g de grasa de sobrepaso; y sin (EGaSg, n = 14) y con (EGaCg, n = 15) la adición de 150 g de grasa de sobrepaso, respectivamente. Se sincronizaron con esponjas de FGA (20 mg), por 12 d, a diez días de su inserción se aplicaron 15 mg de PGF2α. No se encontraron diferencias (P > 0.05) para las... Palavras-chave: Ultrasonografía; Insulina; Progesterona; Estradiol; Hormona luteinizante. Ultrasonagraphy; Insulin; Progesterone; Estradiol; Luteinizing hormone.. Ano: 2012 URL: http://hdl.handle.net/10521/815 Registros recuperados: 63 Primeira ... 1 2 3 4 ... Última

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